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Pir Dependent R6k Origin Of Christmas -- http://shurll.com/bmv4h
Pir Dependent R6k Origin Of Christmas
Conformational changes in a replication origin induced by an initiator protein. However, for a period of several years, π appeared to be unique among Reps in its ability to bind iterons as a dimer (Urh et al., 1998; Diaz-Lopez et al., 2003; Das and Chattoraj, 2004; Diaz-Lopez et al., 2006). Proc Natl Acad Sci U S A. Preponderance of Fis-binding sites in the R6K gamma origin and the curious effect of the penicillin resistance marker on replication of this origin in the absence of Fis. 1993;135:6776. Red circles (purines) and green triangles (pyrimidines) represent bases that affect π binding when removed. This was accomplished by placing pir under the control of a PBAD promoter (from the araBAD operon) and keeping expression levels low (Bowers et al., 2004). Correlation with initiator binding affinity. 1996;257:550560. J Virol. [PMC free article] [PubMed]Uhlin BE, et al. Wu particularly studied the control of replication of R6K. INTRODUCTIONChromosomal and plasmid replicons have evolved strategies to assure their hereditary stability and maintenance at a specific copy number.
Monomerization of RepA dimers by heat shock proteins activates binding to DNA replication origin. Early attempts to crystallize π for structural analysis, however, were thwarted by its tendency to aggregate in solution at high concentrations (Swan et al., 2006 and Filutowicz laboratory, unpublished data), a common property of Rep proteins. 2005;20:833843. These motifs also occur in the DRs of R6K (Fig. Gene. Roughly concurrently, researchers at the Medical University of South Carolina achieved success crystallizing a quadruple mutant of π (Fig. 1978 Sep 25;124(3):443468. A comparison of the sequences of the DRs reveals that only two iterons should be considered 100% consensus, iterons number 2 and 5, whereas the least conserved iteron (number 4) has five bp changes from the consensus 22-bp sequence. Cold Spring Harb Symp Quant Biol. 2004;279:4105841066. The DnaK-DnaJ-GrpE chaperone system activates inert wild type π initiator protein of R6K into a form active in replication initiation. [PubMed]Kunnimalaiyaan S, et al. cf4ac695ea
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